Photon and electron behaviour can only be described when they have both wave and particle properties. In accordance with the quantum theory, a plasmon is the particle name of the electron density waves. Therefore, when in a TIR situation the quantum energy of the photons is right, the photons are converted to plasmons leaving a 'gap' in the reflected light intensity.
A
perfectly matched layer (
PML) is an artificial absorbing layer for
wave equations, commonly used to truncate computational regions in
numerical methods to simulate problems with open boundaries, especially in the
FDTD and
FE methods. The key property of a PML that distinguishes it from an ordinary absorbing material is that it is designed so that waves incident upon the PML from a non-PML medium do not reflect at the interface—this property allows the PML to strongly absorb outgoing waves from the interior of a computational region without reflecting them back into the interior.
PML was originally formulated by Berenger in 1994 for use with
Maxwell's equations, and since that time there have been several related reformulations of PML for both Maxwell's equations and for other wave equations. Berenger's original formulation is called a
split-field PML, because it splits the
electromagnetic fields into two unphysical fields in the PML region. A later formulation that has become more popular because of its simplicity and efficiency is called
uniaxial PML or
UPML (Gedney, 1996), in which the PML is described as an artificial
anisotropic absorbing material. Although both Berenger's formulation and UPML were initially derived by manually constructing the conditions under which incident
plane waves do not reflect from the PML interface from a homogeneous medium,
both formulations were later shown to be equivalent to a much more elegant and general approach:
stretched-coordinate PML (Chew and Weedon, 1994; Teixeira and Chew, 1998). In particular, PMLs were shown to correspond to a
coordinate transformation in which one (or more) coordinates are mapped to
complex numbers; more technically, this is actually an
analytic continuation of the wave equation into complex coordinates, replacing propagating (oscillating) waves by
exponentially decaying waves. This viewpoint allows PMLs to be derived for inhomogeneous media such as
waveguides, as well as for other
coordinate systems and wave equations.
As molecules are immobilized on a sensor surface, the refractive index at the interface between the surface and a solution flowing over the surface changes, altering the angle at which reduced-intensity polarized light is reflected from a supporting glass plane.
The change in angle, caused by binding or dissociation of molecules from the sensor surface, is proportional to the mass of bound material and is recorded in a sensorgram.
When sample is passed over the sensor surface, the sensorgram shows an increasing response as molecules interact. The response remains constant if the interaction reaches equilibrium. When sample is replaced by buffer, the response decreases as the interaction partners dissociate.
Complete profiles of recognition, binding and dissociation are generated in real time. From these profiles, data such as specificity, affinity, kinetic behavior and sample concentration can be determined.
For most applications, a dextran matrix covering the gold layer enables molecules to be immobilized to a sensor surface and provides a hydrophilic environment for interactions. Surface specificity is determined by the nature of the immobilized molecule.
Since light does not penetrate the sample, interactions can be followed in colored, turbid or opaque samples. No labels are required and detection is instantaneous.
This is the full model of SPR generation can be used for the focusing light into very small and thin films, which are smaller as compare to the wavelengths focused inside it.
Gradient
Force: The Gaussian beam has
every angle from -180° to 180° so its gradient have a curve, which
is negative for positive direction and positive for negative
direction on x-axis. So because of Brownian motion of the particle
when it comes in this region of intensity gradient, it is always
forced toward the center of laser beam. This force ,which attract the
particle towards the center of the beam is called as Gradient force.
When this gradient force is sufficiently larger as compared to
scattering force, the particle will be trapped inside the trap
volume.
For
Rayleigh particle, the gradient force can be given as:
Fgrad
= - nb3r3 /2 (m 2-1/ m
2-2) grad(E2)
Thermal
Force:- Mie particles are
moving with Browne motion in the open atmosphere so they possase a
momentum because of their speed. So they are having a force inside
them called as thermal force, since it increases with the
temperature. Sice we are woking in very small particles so small
change in temperature will require change in Gradient force to trap
them.
Thermal
force applied to the particle is
|F(f)|2
= 4γkBT
By
this we can see that force applied to the particle is depending on
the temperature, so if temperature will increase thermal force will
also increase and we have to control it so that particle will be trap
inside the system. The
wave vector because of the Brauny motion is
|X(F)|2
= KBT/
γ(fc-f)2